拟报告大纲 |
报告题目:Over expression and functional analyses of a protein nanocapsule, Encapsulin
摘要:Nanoparticles have been one of the most important key technologies among various scientific fields such as Biotechnology, Chemical Engineering, and Analytical Chemistry. Especially, nanoparticles using gold, quantum dots, and amphiphilic polymers have been extensively studied. Proteins are the most abundant nanomaterials in living organisms and have been considered to have potentially very excellent characteristics in self-organization, chemical activities and structural homogeneity. However, studies treating proteins as nanoparticles have been very limited except for some pioneering researches on ferritin and peripheral virus particles, because of their complex structures, difficulties in self-organization and/or assembly, and instability against temperature, pH and organic solvents. We have been studied a nanoparticle-forming protein, Encapsulin. It has a molecular weight of about 30k, and 60 copies of the Encapsulin subunit form a spherical particle with a thin T=1 icosahedral shell with a diameter of 24 nm (1). Here, we show two kinds of Encapsulins from a mesophilic bacterium, Rhodococcus erythropolis N771 and a hyperthermophilic one, Thermotoga maritima.
|